The subcellular location of ErGPCR-2 was analyzed to observe its rapid response to 20E induction. ErGPCR-2 protein was mainly localized in the cell membrane, ovinum pantip. However, ErGPCR-2 was internalized into the cytoplasm within 15 min of 20E treatment (Figure 5A). Western blot analysis confirmed the internalization of ErGPCR-2 by 20E induction. The molecular mass of ErGPCR-2 in the cytoplasm was also higher than that in the cell membrane (Figure 5B).
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https://readbook.cz/groups/create/step/docs/ However, no difference existed on the [ 3 H]Pon A levels in co-immunoprecipitates from normal cells and dsGFP- or dsErGPCR-2 -treated cells (Figure 7B), suggesting that ErGPCR-2 does not bind to [ 3 H]Pon A in the cell membrane or in the cytosol, ovinum pantip.
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